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Volume :9 Issue : 1 1982      Add To Cart                                                                    Download

Optimisation of cortisol radioimmunoassay in serum

Auther : Y. M. MEGAHED,G. A. ZIADA, M. M. MAHFOUZ,M.ABDEL-RAZZAK AND S. E. EL-HADDAD

Radioisotope Department, Atomic Energy Establishment, Cairo, and College of Medicine, Cairo.University Egypt

 

ABSTRACT

 

An optimised method for accurate radioimmunoassay of cortisol in serum has been developed. The method involved inhibition of binding of cortisol to protein using a TCA/NaOH mixture. The antibodies used were obtained from Mallinkrodt. The separation technique used was that of dextran charcoal which adsorbs free cortisol. The kinetics of antibody-antigen combination, and the conditions of the experiment including charcoal concentration, antibody dilution, temperature, time of incubation and radioactivity concentration were studied to optimise the standard curve and to obtain high reproducibility; the variance coefficient was evaluated. Serum samples of different patients were investigated, and the results compared with that of protein binding analysis from 35 normals and 35 hypotensive patients in fasting and non-fasting conditions. Cortisol concentration increased in both instances after ACTH injection.

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