Kuwait-University-Journal-of-Law-header
Search
Kuwait Journal of Science

Previous Issues

Advance Search
Year : From To Vol
Issue Discipline:
Author

Volume :39 Issue : 1 2012      Add To Cart                                                                    Download

Optimization of bacterial fatty acid methyl esters separation by gas chromatography - mass spectrometry

Auther : N.E. AL-BELOSHI, H. AL-AWADHI, R.A. AL-KHALAF, J. JACQUILION, S. OOMMEN AND M. AFZAL*

 

Department of Biological Sciences, Faculty of Science, Kuwait University, Kuwait
N.E. Albeloshi: NE1978@live.com, H Al-Awadhihusain.alawadhi@ku.edu.kw, R.A. Alkhalaf: omfahad04@hotmail.com,J.Jacquilionjacquilion@yahoo.com, S. Oommen: jubintom@hotmail.com
 
*Corresponding author: M. Afzal, email: Afzalq8@gmail.com                            
                                                                       
 
ABSTRACT
Analysis of bacterial fatty acids as their methyl esters (FAMEs) has been extensively used for rapid diagnosis of anaerobic infections, bacterial classification and taxonomy. This makes bacterial FAMEs and especially hydroxy FAMEs analyses of primary importance for clinical and taxonomical interests. However, selection of the appropriate column and other chromatographic parameters makes it an intricate issue for the simultaneous separation of various classes of compounds. For the resolution of these complex mixtures, we have compared four bonded capillary columns OV1, HP-INNOwax, DB5, and VF5-ms of the same length, internal diameter and coated film thickness. Experimental conditions such as gas flow rate and oven temperature programming were optimized to achieve the best resolution on these columns. The standard FAMEs used in this study contained 6 hydroxy fatty acids, two geometric isomers, two positional isomers in branch chain, a cyclopropane and short and long chain components. We have found that OV1 capillary column of low polarity could give best separation of all classes of bacterial FAMEs present in a mixture. Identification of the FAMEs was carried out from their mass spectral fragmentation.
 
Keywords: Bacterial fatty acids; branch chain fatty acids; capillary columns; FAMEs, gas chromatography; hydroxy-FAMEs; mass spectrometry;
 

Kuwait Journal of Science
Journal of Law

You are Visitor No.

55658

Journal of Law
Journal of Law
Tell your friendsJournal of Law
Journal of Law

Last Updated

Jun 19, 2012

Journal of Law
Journal of Law
Journal of Law

Please enter your email Here to receive our news

Journal of Law