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Volume :28 Issue : 2 2001      Add To Cart                                                                    Download

Nutritional controls of proteolytic and keratinolytic activities by a keratinophilic Chrysosporium from soil in Kuwait

Auther : A. A. AL-MUSALLAM*, N. A. AL-SANE AND S. S. AL-ZARBAN

Biological Sciences Department, Kuwait University, P.O. Box 5969, 13060 Safat, Kuwait 

*e-mail: azza@kuc01.kuniv.edu.kw  

ABSTRACT 

Proteolytic activity of Chrysosporium zonatum, which is a keratinophilic species from soil, has been expressed in the exponential growth phase in Sabouraude dextrose broth (SDB). The four-week-old stationary grown mats were transferred into different minimal media that had been depleted from either carbon, nitrogen, sulfur or phosphorus. These mats expressed higher proteolytic activities at day seven. Addition of keratin as the sole source of carbon and nitrogen further enhanced proteolytic and keratinolytic activity.

Keywords: C. zonatum; catabolite repression; dermatophytes; keratinase; protease.

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