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Volume :26 Issue : 1 1999      Add To Cart                                                                    Download

A single DNA of tomato yellow leaf curl geminivirus causing epidemics in the State of Kuwait

Auther : MS. MONTASSER*, A. AL-SHARIDAH*, N.Y. ALI*, M.K. NAKHLA**, B.L. FARAG** AND D.P. MAXWELL

* Department of Biological Sciences, Faculty of Science, University of Kuwait, P. 0. Box 5969, Safat 13060, State of Kuwait

** Department of Plant Pathology, Russell Laboratories, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, WI 53706-1598, USA

 

ABSTRACT

A naturally occurring viral disease has been devastating field-grown tomato (Lycopersicon esculentum Mill.) crops in Kuwait. The disease outbreak was observed during the growing seasons of 1993 through 1999 causing a major loss in tomato fruit yield. Host range, symptomatology, and insect transmission studies showed that the causal agent was transmitted by the whitefly, Bemisia tabaci Genn. Based on electron microscopy, dot blot hybridization, and polymerase chain reaction (PCR), the causal virus was identified as tomato yellow leaf curl virus (TYLCV). All samples collected from several vegetable gardens and fields including plastic-tunnels, protected cultivations and greenhouses were almost 100% infected. Using degenerate primer pairs designed for PCR amplification of both viral genomic DNA-A and DNA-B component sequences, about 1-kb DNA product was consistently amplified from infected tomato samples. PCR-amplified viral fragments were further characterized and confirmed by Southern blot hybridization analysis. No reactions were obtained with the degenerate primer set designed for DNA-B amplification or with the positive control of DNA-B using primer sets for DNA-A components The results revealed that the causal agent of the disease was an Eastern Mediterranean strain of the monopartite geminivirus possessing a single genomic DNA-A component and hence designated as TYLCV-K.

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