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Volume :22 Issue : 1 1995      Add To Cart                                                                    Download

Cytochemical localization of acid phosphatase activity(cerium-based method) in the proximal convoluted tubule of the kidney of the gerbil Meriones crassus with reference to theGERL complex and lysosomal-vacuolar apparatus

Auther : ABDEL-MAJEED A. SAFER

 Zoology Department, Kuwait University, P.O. Box 5969, Sq/at 13060, Kuwait

 ABSTRACT

Ultrastructural localizations of acid phosphatase activity were established in the proximal convoluted tubule cells of the gerbil Meriones crassus using â-glycerophosphate as a substrate and cerium chloride as a capture agent. The reaction product of the cerium phosphate is finely precipitated in different sites of the Golgi associated Endoplasmic Reticulum involved in the formation of Lysosomes (GERL) complex including its cisternal and tubular elements, and the lysosomal-vacuolar apparatus. Prominent connection between GERL elements and vesiculated lysosomes was found, indicating the possible origin of the primary lysosome. However, the GERL elements are numerous and extensive through the matrices of the cells with different patterns and textures located, as usual, in the vicinity of the nuclei and the Golgi apparatus. The Study confirms the presence of GERL elements similar to that seen in related studies. The Golgi apparatus and the apical surface of the cells showed no reaction. Neither the basal infoldings nor the membrane-bound bodies of the thick basal lamina showed any activity, and this may cast doubt upon the lysosomal origin of these bodies. The enzymatic activity also indicates heavy deposits of acid phosphatase-positive lysosomes which are distributed as regular, pherica1 to irregular structures. Some appear dumbbell-shaped, others contain inclusions, while still others show remarkable fusion activity. The fine cerium depositions illustrate the c in the GERL elements which when tilted at few degrees from the flat, show their nastomosing pattern on the one hand, and their parallel tubular elements on the other. It also shows the clear detection of the peripheral space between the limiting membrane of the lysosome and its stained contents, as well as the connection of some lysosomes to each other with a thread-like structure.

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