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Faculty of agriculture and Bilogy, Nuclear Research Center, P.O. Box: 765, Baghdad, Iraq.

 

 

Pollen of Citrus aurantium L. irradiated with various doses of gamma rays was used to pollinate C. sinesnsis (L). Osbeck flowers. Polyembryogenesis was repressed in the hybrid ovules when cultured on nutrient medium enriched with prescribed concentrations of specific growth regulators.  More repression was achieved through direct irradiation of the ovules, rather than the pollen.  Identification of hybrid seedlings was achieved through isozyme analysis of leaf extracts on 12% polyarcrylamide gel.  Leucine aminopeptidase (LAP) was detected in 6 molecular forms in the hybrid seedlings.  Analyses of LAP isozymes in all developed seedlings showed a hybrid banding pattern, suggesting their zygotic origin.  Thus the inhibition was selective to nucellar embryos.