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Department of Chemistry, College of Science, University of Mosul, Mosul, Iraq

ABSTRACT

A sensitive and accurate titrimetric method with an amplification procedure for the determination of 10-1,000 mg of cysteine and 50-2,000 mg of glutathione is presented. It is based on oxidation of an acidic sample solution of cysteine or glutathione with a chloroform solution of iodine, removal of its excess, oxidation of the resulting iodide with bromine, and iodo metric titration of the iodate produced. The coefficients of variation range from 0.1 to 1.5%, depending on the concentration levels of cysteine and glutathione.