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Volume :16 Issue : 1 1989      Add To Cart                                                                    Download

Tissue culture of Rhazya stricta Decaisne

Auther : MOBASHER S. OMAR

Faculty of Agriculture and Biology, Nuclear Research Center, P.O. Box 765, Baghdad, Iraq.

ABSTRACT

Seeds of Rhazya stricta Decaisne were germinated in nutrient medium enriched with gibberellic acid (GA,). The auxin indoleacetic acid (IAA) stimulated profuse callus initiation in excised roots segments, and abundant root formation. Naphthalenacetic acid (NAA) promoted soft friable callus in stem and leaf segments, and 2,4 dichlorophenoxyacetic acid (2,4-D) in cotyledons as well as leaf segments. Different combinations of various growth regulating substances promoted callus development. Plant regeneration was obtained from stem-derived callus grown in medium containing NAA. Preliminary investigation of calli extracts suggested the presence of alkaloids.

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